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rabbit antihuman ap 2a polyclonal antibodies  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology rabbit antihuman ap 2a polyclonal antibodies
    Figure1. Expression of <t>AP-2a</t> (red £uorescence) during di¡erent stages of HF morphogenesis in neonatal mouse skin (days 0^5 postpartum). Cryostat sections (10 mm) were incubated with primary rabbit antihuman AP-2a <t>polyclonal</t> antibodies and then treated with rhodamine-conjugated F(ab)2 fragments of a goat antirabbit IgG. (A) Stage 1 of HF morphogenesis. High AP-2a IR is seen in the epithelial placode. Mesenchymal condensation beneath the follicle plug is slightly positive (arrow). (B)^(C) Stage 2. Some suprabasal epidermal keratinocytes above the placode are highly positive as well (arrow- heads). (D) Stage 3. The entire hair plug becomes AP-2a positive while engul¢ng the FP (arrow). (E) Stage 4.With progression of the downward growth of the HF, AP-2a expression declined in the lower portion but remained high in the follicle infundibulum. (F) Stage 5. Three zones of AP-2a IR are formed in the HF: the uppermost HF portion (infundibulum) (1), the zone of sebocyte di¡erentiation (2), and a newly formed morphogenic zone that gives rise to the IRS (3). (G) With initiation of formation of ascending layers of HF, AP-2a expression is most prominent in the IRS cone (white arrowhead). (H) The early formation of the hair shaft and invasion of the HF into the dermis (stages 5^6 of morphogenesis) were associated with an increase of AP-2a IR in FP cells. (I) The sebaceous glands, the epithelial lining of the HF infundibulum, and the basal layer of interfollicular epidermis remained persistently AP-2a positive. be, basal layer of epidermis; ORS, outer root sheath; sg, sebaceous gland. Scale bars: (A)^(E), (H) 23 mm; (F), (G), (I) 35 mm.
    Rabbit Antihuman Ap 2a Polyclonal Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+antihuman+ap+2a+polyclonal+antibodies/Rab+2A+Antibody/pm12839558-61-8-16
    Average 93 stars, based on 39 article reviews
    rabbit antihuman ap 2a polyclonal antibodies - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Expression patterns of the transcription factor AP-2alpha during hair follicle morphogenesis and cycling."

    Article Title: Expression patterns of the transcription factor AP-2alpha during hair follicle morphogenesis and cycling.

    Journal: The Journal of investigative dermatology

    doi: 10.1046/j.1523-1747.2003.12319.x

    Figure1. Expression of AP-2a (red £uorescence) during di¡erent stages of HF morphogenesis in neonatal mouse skin (days 0^5 postpartum). Cryostat sections (10 mm) were incubated with primary rabbit antihuman AP-2a polyclonal antibodies and then treated with rhodamine-conjugated F(ab)2 fragments of a goat antirabbit IgG. (A) Stage 1 of HF morphogenesis. High AP-2a IR is seen in the epithelial placode. Mesenchymal condensation beneath the follicle plug is slightly positive (arrow). (B)^(C) Stage 2. Some suprabasal epidermal keratinocytes above the placode are highly positive as well (arrow- heads). (D) Stage 3. The entire hair plug becomes AP-2a positive while engul¢ng the FP (arrow). (E) Stage 4.With progression of the downward growth of the HF, AP-2a expression declined in the lower portion but remained high in the follicle infundibulum. (F) Stage 5. Three zones of AP-2a IR are formed in the HF: the uppermost HF portion (infundibulum) (1), the zone of sebocyte di¡erentiation (2), and a newly formed morphogenic zone that gives rise to the IRS (3). (G) With initiation of formation of ascending layers of HF, AP-2a expression is most prominent in the IRS cone (white arrowhead). (H) The early formation of the hair shaft and invasion of the HF into the dermis (stages 5^6 of morphogenesis) were associated with an increase of AP-2a IR in FP cells. (I) The sebaceous glands, the epithelial lining of the HF infundibulum, and the basal layer of interfollicular epidermis remained persistently AP-2a positive. be, basal layer of epidermis; ORS, outer root sheath; sg, sebaceous gland. Scale bars: (A)^(E), (H) 23 mm; (F), (G), (I) 35 mm.
    Figure Legend Snippet: Figure1. Expression of AP-2a (red £uorescence) during di¡erent stages of HF morphogenesis in neonatal mouse skin (days 0^5 postpartum). Cryostat sections (10 mm) were incubated with primary rabbit antihuman AP-2a polyclonal antibodies and then treated with rhodamine-conjugated F(ab)2 fragments of a goat antirabbit IgG. (A) Stage 1 of HF morphogenesis. High AP-2a IR is seen in the epithelial placode. Mesenchymal condensation beneath the follicle plug is slightly positive (arrow). (B)^(C) Stage 2. Some suprabasal epidermal keratinocytes above the placode are highly positive as well (arrow- heads). (D) Stage 3. The entire hair plug becomes AP-2a positive while engul¢ng the FP (arrow). (E) Stage 4.With progression of the downward growth of the HF, AP-2a expression declined in the lower portion but remained high in the follicle infundibulum. (F) Stage 5. Three zones of AP-2a IR are formed in the HF: the uppermost HF portion (infundibulum) (1), the zone of sebocyte di¡erentiation (2), and a newly formed morphogenic zone that gives rise to the IRS (3). (G) With initiation of formation of ascending layers of HF, AP-2a expression is most prominent in the IRS cone (white arrowhead). (H) The early formation of the hair shaft and invasion of the HF into the dermis (stages 5^6 of morphogenesis) were associated with an increase of AP-2a IR in FP cells. (I) The sebaceous glands, the epithelial lining of the HF infundibulum, and the basal layer of interfollicular epidermis remained persistently AP-2a positive. be, basal layer of epidermis; ORS, outer root sheath; sg, sebaceous gland. Scale bars: (A)^(E), (H) 23 mm; (F), (G), (I) 35 mm.

    Techniques Used: Expressing, Incubation

    Related Articles

    Incubation:

    Article Title: Expression patterns of the transcription factor AP-2alpha during hair follicle morphogenesis and cycling.
    Article Snippet: For AP-2a immunohistochemistry, acetone-¢xed frozen sections were presoaked inTris-bu¡ered saline and successively treated with 10% normal goat serum (Dako). .. After the overnight incubation (room temperature) with primary rabbit antihuman AP-2a polyclonal antibodies (sc-184, clone C-18; Santa Cruz Biotechnology, Santa Cruz, CA) at a dilution of 1:500, the sections were treated with rhodamine-conjugated F(ab)2 fragments of a goat antirabbit IgG (Jackson ImmunoResearch,West Grove, PA) at a dilution of 1:200 (1 h; 371C), counterstained with Hoechst 33342 dye (10 mg per ml) (Sigma, St. Louis, MO), and mounted with Vectashield H-1000 (Vector, Burlingame, CA). ..



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    rabbit antihuman ap 2a polyclonal antibodies  (Santa Cruz Biotechnology)
    93
    Santa Cruz Biotechnology rabbit antihuman ap 2a polyclonal antibodies
    Figure1. Expression of <t>AP-2a</t> (red £uorescence) during di¡erent stages of HF morphogenesis in neonatal mouse skin (days 0^5 postpartum). Cryostat sections (10 mm) were incubated with primary rabbit antihuman AP-2a <t>polyclonal</t> antibodies and then treated with rhodamine-conjugated F(ab)2 fragments of a goat antirabbit IgG. (A) Stage 1 of HF morphogenesis. High AP-2a IR is seen in the epithelial placode. Mesenchymal condensation beneath the follicle plug is slightly positive (arrow). (B)^(C) Stage 2. Some suprabasal epidermal keratinocytes above the placode are highly positive as well (arrow- heads). (D) Stage 3. The entire hair plug becomes AP-2a positive while engul¢ng the FP (arrow). (E) Stage 4.With progression of the downward growth of the HF, AP-2a expression declined in the lower portion but remained high in the follicle infundibulum. (F) Stage 5. Three zones of AP-2a IR are formed in the HF: the uppermost HF portion (infundibulum) (1), the zone of sebocyte di¡erentiation (2), and a newly formed morphogenic zone that gives rise to the IRS (3). (G) With initiation of formation of ascending layers of HF, AP-2a expression is most prominent in the IRS cone (white arrowhead). (H) The early formation of the hair shaft and invasion of the HF into the dermis (stages 5^6 of morphogenesis) were associated with an increase of AP-2a IR in FP cells. (I) The sebaceous glands, the epithelial lining of the HF infundibulum, and the basal layer of interfollicular epidermis remained persistently AP-2a positive. be, basal layer of epidermis; ORS, outer root sheath; sg, sebaceous gland. Scale bars: (A)^(E), (H) 23 mm; (F), (G), (I) 35 mm.
    Rabbit Antihuman Ap 2a Polyclonal Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+antihuman+ap+2a+polyclonal+antibodies/Rab+2A+Antibody/pm12839558-61-8-16
    Average 93 stars, based on 1 article reviews
    rabbit antihuman ap 2a polyclonal antibodies - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure1. Expression of AP-2a (red £uorescence) during di¡erent stages of HF morphogenesis in neonatal mouse skin (days 0^5 postpartum). Cryostat sections (10 mm) were incubated with primary rabbit antihuman AP-2a polyclonal antibodies and then treated with rhodamine-conjugated F(ab)2 fragments of a goat antirabbit IgG. (A) Stage 1 of HF morphogenesis. High AP-2a IR is seen in the epithelial placode. Mesenchymal condensation beneath the follicle plug is slightly positive (arrow). (B)^(C) Stage 2. Some suprabasal epidermal keratinocytes above the placode are highly positive as well (arrow- heads). (D) Stage 3. The entire hair plug becomes AP-2a positive while engul¢ng the FP (arrow). (E) Stage 4.With progression of the downward growth of the HF, AP-2a expression declined in the lower portion but remained high in the follicle infundibulum. (F) Stage 5. Three zones of AP-2a IR are formed in the HF: the uppermost HF portion (infundibulum) (1), the zone of sebocyte di¡erentiation (2), and a newly formed morphogenic zone that gives rise to the IRS (3). (G) With initiation of formation of ascending layers of HF, AP-2a expression is most prominent in the IRS cone (white arrowhead). (H) The early formation of the hair shaft and invasion of the HF into the dermis (stages 5^6 of morphogenesis) were associated with an increase of AP-2a IR in FP cells. (I) The sebaceous glands, the epithelial lining of the HF infundibulum, and the basal layer of interfollicular epidermis remained persistently AP-2a positive. be, basal layer of epidermis; ORS, outer root sheath; sg, sebaceous gland. Scale bars: (A)^(E), (H) 23 mm; (F), (G), (I) 35 mm.

    Journal: The Journal of investigative dermatology

    Article Title: Expression patterns of the transcription factor AP-2alpha during hair follicle morphogenesis and cycling.

    doi: 10.1046/j.1523-1747.2003.12319.x

    Figure Lengend Snippet: Figure1. Expression of AP-2a (red £uorescence) during di¡erent stages of HF morphogenesis in neonatal mouse skin (days 0^5 postpartum). Cryostat sections (10 mm) were incubated with primary rabbit antihuman AP-2a polyclonal antibodies and then treated with rhodamine-conjugated F(ab)2 fragments of a goat antirabbit IgG. (A) Stage 1 of HF morphogenesis. High AP-2a IR is seen in the epithelial placode. Mesenchymal condensation beneath the follicle plug is slightly positive (arrow). (B)^(C) Stage 2. Some suprabasal epidermal keratinocytes above the placode are highly positive as well (arrow- heads). (D) Stage 3. The entire hair plug becomes AP-2a positive while engul¢ng the FP (arrow). (E) Stage 4.With progression of the downward growth of the HF, AP-2a expression declined in the lower portion but remained high in the follicle infundibulum. (F) Stage 5. Three zones of AP-2a IR are formed in the HF: the uppermost HF portion (infundibulum) (1), the zone of sebocyte di¡erentiation (2), and a newly formed morphogenic zone that gives rise to the IRS (3). (G) With initiation of formation of ascending layers of HF, AP-2a expression is most prominent in the IRS cone (white arrowhead). (H) The early formation of the hair shaft and invasion of the HF into the dermis (stages 5^6 of morphogenesis) were associated with an increase of AP-2a IR in FP cells. (I) The sebaceous glands, the epithelial lining of the HF infundibulum, and the basal layer of interfollicular epidermis remained persistently AP-2a positive. be, basal layer of epidermis; ORS, outer root sheath; sg, sebaceous gland. Scale bars: (A)^(E), (H) 23 mm; (F), (G), (I) 35 mm.

    Article Snippet: After the overnight incubation (room temperature) with primary rabbit antihuman AP-2a polyclonal antibodies (sc-184, clone C-18; Santa Cruz Biotechnology, Santa Cruz, CA) at a dilution of 1:500, the sections were treated with rhodamine-conjugated F(ab)2 fragments of a goat antirabbit IgG (Jackson ImmunoResearch,West Grove, PA) at a dilution of 1:200 (1 h; 371C), counterstained with Hoechst 33342 dye (10 mg per ml) (Sigma, St. Louis, MO), and mounted with Vectashield H-1000 (Vector, Burlingame, CA).

    Techniques: Expressing, Incubation